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Check what kind of vein (e.g. jugular or cephalic) is best to use for a blood collection. The vein is the narrowest part of an artery. The easiest and most common type of vein to use is a vein that is close in proximity to the aorta. Try to insert the needle in this kind of vein, and make sure to be in a horizontal position before the needle is inserted.
If you manage to get the blood sample, you will need to label it and send it on time. If blood collection is scheduled far in advance, you may save a considerable amount of blood collection attempts. You may need to use only one needle as well as the needle holder.
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The HPA or heparinised syringe used to collect blood samples must be filled with heparin or saline solution up to the very top or filled to 1 ml, for a maximum volume of 10 ml); to collect blood, 0.5 ml of full blood should be removed from the needle, and about two-thirds of the syringe has to be drawn back and released into the sample tube. d2c66b5586