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The distribution of zinc in ferret adrenal cytoplasm was investigated by means of X-ray microanalysis using biological standard materials. We focused on the difference in concentration of zinc in the cytoplasm of adrenocortical cells of female and male ferrets at various stages of maturation. In female ferrets at different stages of maturation, the absolute concentration of zinc in cytoplasm increased gradually during the period of maturation. In male ferrets, the cytoplasm of adrenocortical cells contained only small amounts of zinc. Furthermore, in adult male ferrets, the amount of zinc was significantly lower than that in females of the same age. In the cytoplasm of adrenocortical cells of female ferrets at different stages of maturation, the absolute concentration of zinc increased gradually. However, in those of male ferrets, it was lower than that of females. The amount of zinc was highest in adult ferrets. The concentration of zinc in the cytoplasm of adrenocortical cells of female ferrets was estimated to be 12.7 +/- 1.3 nmol/g wet weight (mean +/- SE) and that of male ferrets was 1.1 +/- 0.2 nmol/g wet weight. Zinc was co-distributed with manganese, iron and copper in the cytoplasm of adrenocortical cells. The study suggests that the male ferret may lack adrenocortical androgens in adrenal cytoplasm because of the low concentration of zinc in the cytoplasm of adrenocortical cells.Swedish House Mafia and The Knife’s Tommie Sunshine spin the Grammy clock “We have nothing to prove,” says Swedish House Mafia at the start of their hour-long performance. “We have been playing together a long time, and we know each other,” says Knife� 0b46394aab